entry created 2009-04-14 dataset Swiss-Prot modified 2023-02-22 version 67 accession B7N8U0 name AROL_ECOLU protein recommendedName fullName evidence 1 Shikimate-kinase-2 shortName evidence 1 SK-2 ecNumber evidence 1 2.7.1.71 gene name evidence 1 type primary aroL name type ordered-locus ECUMN_0426 organism name type scientific Escherichia-coli-O17:K52:H18-(strain-UMN026-/-ExPEC) dbReference id 585056 type NCBI-Taxonomy lineage taxon Bacteria taxon Proteobacteria taxon Gammaproteobacteria taxon Enterobacterales taxon Enterobacteriaceae taxon Escherichia reference key 1 citation date 2009 first E1000344 last E1000344 name PLoS-Genet. type journal-article volume 5 title Organised-genome-dynamics-in-the-Escherichia-coli-species-results-in-highly-diverse-adaptive-paths. authorList person name Touchon-M. person name Hoede-C. person name Tenaillon-O. person name Barbe-V. person name Baeriswyl-S. person name Bidet-P. person name Bingen-E. person name Bonacorsi-S. person name Bouchier-C. person name Bouvet-O. person name Calteau-A. person name Chiapello-H. person name Clermont-O. person name Cruveiller-S. person name Danchin-A. person name Diard-M. person name Dossat-C. person name Karoui-M.E. person name Frapy-E. person name Garry-L. person name Ghigo-J.M. person name Gilles-A.M. person name Johnson-J. person name Le-Bouguenec-C. person name Lescat-M. person name Mangenot-S. person name Martinez-Jehanne-V. person name Matic-I. person name Nassif-X. person name Oztas-S. person name Petit-M.A. person name Pichon-C. person name Rouy-Z. person name Ruf-C.S. person name Schneider-D. person name Tourret-J. person name Vacherie-B. person name Vallenet-D. person name Medigue-C. person name Rocha-E.P.C. person name Denamur-E. dbReference id 19165319 type PubMed dbReference id 10.1371/journal.pgen.1000344 type DOI scope NUCLEOTIDE-SEQUENCE-[LARGE-SCALE-GENOMIC-DNA] source strain UMN026-/-ExPEC comment type function text evidence 1 Catalyzes-the-specific-phosphorylation-of-the-3-hydroxyl-group-of-shikimic-acid-using-ATP-as-a-cosubstrate. comment type catalytic-activity reaction evidence 1 text ATP-+-shikimate-=-3-phosphoshikimate-+-ADP-+-H(+) dbReference id RHEA:13121 type Rhea dbReference id CHEBI:15378 type ChEBI dbReference id CHEBI:30616 type ChEBI dbReference id CHEBI:36208 type ChEBI dbReference id CHEBI:145989 type ChEBI dbReference id CHEBI:456216 type ChEBI dbReference id 2.7.1.71 type EC comment type cofactor cofactor evidence 1 name Mg(2+) dbReference id CHEBI:18420 type ChEBI text evidence 1 Binds-1-Mg(2+)-ion-per-subunit. comment type pathway text evidence 1 Metabolic-intermediate-biosynthesis;-chorismate-biosynthesis;-chorismate-from-D-erythrose-4-phosphate-and-phosphoenolpyruvate:-step-5/7. comment type subunit text evidence 1 Monomer. comment type subcellular-location subcellularLocation location evidence 1 Cytoplasm comment type domain text evidence 1 The-LID-domain-closes-over-the-active-site-upon-ATP-binding. comment type similarity text evidence 1 Belongs-to-the-shikimate-kinase-family.-AroL-subfamily. dbReference evidence 1 id 2.7.1.71 type EC dbReference id CU928163 type EMBL property type protein-sequence-ID value CAR11641.1 property type molecule-type value Genomic_DNA dbReference id WP_000193393.1 type RefSeq property type nucleotide-sequence-ID value NC_011751.1 dbReference id YP_002411189.1 type RefSeq property type nucleotide-sequence-ID value NC_011751.1 dbReference id B7N8U0 type AlphaFoldDB dbReference id B7N8U0 type SMR dbReference id 585056.ECUMN_0426 type STRING dbReference id CAR11641 type EnsemblBacteria property type protein-sequence-ID value CAR11641 property type gene-ID value ECUMN_0426 dbReference id 66671313 type GeneID dbReference id eum:ECUMN_0426 type KEGG dbReference id fig|585056.7.peg.625 type PATRIC dbReference id CLU_057607_4_3_6 type HOGENOM dbReference id DTDIFMQ type OMA dbReference id UPA00053 type UniPathway property type reaction-ID value UER00088 dbReference id UP000007097 type Proteomes property type component value Chromosome dbReference id GO:0005737 type GO property type term value C:cytoplasm property type evidence value ECO:0007669 property type project value UniProtKB-SubCell dbReference id GO:0005524 type GO property type term value F:ATP-binding property type evidence value ECO:0007669 property type project value UniProtKB-UniRule dbReference id GO:0000287 type GO property type term value F:magnesium-ion-binding property type evidence value ECO:0007669 property type project value UniProtKB-UniRule dbReference id GO:0004765 type GO property type term value F:shikimate-kinase-activity property type evidence value ECO:0007669 property type project value UniProtKB-UniRule dbReference id GO:0008652 type GO property type term value P:amino-acid-biosynthetic-process property type evidence value ECO:0007669 property type project value UniProtKB-KW dbReference id GO:0009073 type GO property type term value P:aromatic-amino-acid-family-biosynthetic-process property type evidence value ECO:0007669 property type project value UniProtKB-UniRule dbReference id GO:0009423 type GO property type term value P:chorismate-biosynthetic-process property type evidence value ECO:0007669 property type project value UniProtKB-UniPathway dbReference id GO:0016310 type GO property type term value P:phosphorylation property type evidence value ECO:0007669 property type project value UniProtKB-KW dbReference id cd00464 type CDD property type entry-name value SK property type match-status value 1 dbReference id 3.40.50.300 type Gene3D property type entry-name value P-loop-containing-nucleotide-triphosphate-hydrolases property type match-status value 1 dbReference id MF_00109 type HAMAP property type entry-name value Shikimate_kinase property type match-status value 1 dbReference id MF_01269 type HAMAP property type entry-name value Shikimate_kinase_2 property type match-status value 1 dbReference id IPR027417 type InterPro property type entry-name value P-loop_NTPase dbReference id IPR031322 type InterPro property type entry-name value Shikimate/glucono_kinase dbReference id IPR000623 type InterPro property type entry-name value Shikimate_kinase/TSH1 dbReference id IPR027544 type InterPro property type entry-name value Shikimate_kinase_2 dbReference id IPR023000 type InterPro property type entry-name value Shikimate_kinase_CS dbReference id PTHR21087 type PANTHER property type entry-name value SHIKIMATE-KINASE property type match-status value 1 dbReference id PTHR21087:SF21 type PANTHER property type entry-name value SHIKIMATE-KINASE-2 property type match-status value 1 dbReference id PF01202 type Pfam property type entry-name value SKI property type match-status value 1 dbReference id PR01100 type PRINTS property type entry-name value SHIKIMTKNASE dbReference id SSF52540 type SUPFAM property type entry-name value P-loop-containing-nucleoside-triphosphate-hydrolases property type match-status value 1 dbReference id PS01128 type PROSITE property type entry-name value SHIKIMATE_KINASE property type match-status value 1 proteinExistence type inferred-from-homology keyword id KW-0028 Amino-acid-biosynthesis keyword id KW-0057 Aromatic-amino-acid-biosynthesis keyword id KW-0067 ATP-binding keyword id KW-0963 Cytoplasm keyword id KW-0418 Kinase keyword id KW-0460 Magnesium keyword id KW-0479 Metal-binding keyword id KW-0547 Nucleotide-binding keyword id KW-0808 Transferase feature description Shikimate-kinase-2 id PRO_1000140132 type chain location begin position 1 end position 174 feature description LID-domain type region-of-interest location begin position 112 end position 126 feature evidence 1 type binding-site location begin position 12 end position 17 ligand name ATP dbReference id CHEBI:30616 type ChEBI feature evidence 1 type binding-site location position position 16 ligand name Mg(2+) dbReference id CHEBI:18420 type ChEBI feature evidence 1 type binding-site location position position 32 ligand name Mg(2+) dbReference id CHEBI:18420 type ChEBI feature evidence 1 type binding-site location position position 34 ligand name substrate feature evidence 1 type binding-site location position position 58 ligand name substrate feature evidence 1 type binding-site location position position 79 ligand name substrate feature evidence 1 type binding-site location position position 120 ligand name ATP dbReference id CHEBI:30616 type ChEBI feature evidence 1 type binding-site location position position 139 ligand name substrate evidence key 1 type ECO:0000255 source dbReference id MF_01269 type HAMAP-Rule sequence checksum FCB8D86F6DD55347 length 174 mass 19151 modified 2009-03-24 version 1 MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMTVAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCAPVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQVISEIRSALAQTINC 
entry created 2009-04-14 dataset Swiss-Prot modified 2023-02-22 version 68 accession B7HL50 name CCA_BACC7 protein recommendedName fullName evidence 1 CCA-adding-enzyme ecNumber evidence 1 2.7.7.72 alternativeName fullName evidence 1 CCA-tRNA-nucleotidyltransferase alternativeName fullName evidence 1 tRNA-CCA-pyrophosphorylase alternativeName fullName evidence 1 tRNA-adenylyl-/cytidylyl--transferase alternativeName fullName evidence 1 tRNA-nucleotidyltransferase alternativeName fullName evidence 1 tRNA-NT gene name evidence 1 type primary cca name type ordered-locus BCAH187_A1703 organism name type scientific Bacillus-cereus-(strain-AH187) dbReference id 405534 type NCBI-Taxonomy lineage taxon Bacteria taxon Firmicutes taxon Bacilli taxon Bacillales taxon Bacillaceae taxon Bacillus taxon Bacillus-cereus-group reference key 1 citation date 2008-10 db EMBL/GenBank/DDBJ-databases type submission title Genome-sequence-of-Bacillus-cereus-AH187. authorList person name Dodson-R.J. person name Durkin-A.S. person name Rosovitz-M.J. person name Rasko-D.A. person name Kolsto-A.B. person name Okstad-O.A. person name Ravel-J. person name Sutton-G. scope NUCLEOTIDE-SEQUENCE-[LARGE-SCALE-GENOMIC-DNA] source strain AH187 comment type function text evidence 1 Catalyzes-the-addition-and-repair-of-the-essential-3'-terminal-CCA-sequence-in-tRNAs-without-using-a-nucleic-acid-template.-Adds-these-three-nucleotides-in-the-order-of-C,-C,-and-A-to-the-tRNA-nucleotide-73,-using-CTP-and-ATP-as-substrates-and-producing-inorganic-pyrophosphate. comment type catalytic-activity reaction evidence 1 text a-tRNA-precursor-+-ATP-+-2-CTP-=-a-tRNA-with-a-3'-CCA-end-+-3-diphosphate dbReference id RHEA:14433 type Rhea dbReference id RHEA-COMP:10465 type Rhea dbReference id RHEA-COMP:10468 type Rhea dbReference id CHEBI:30616 type ChEBI dbReference id CHEBI:33019 type ChEBI dbReference id CHEBI:37563 type ChEBI dbReference id CHEBI:74896 type ChEBI dbReference id CHEBI:83071 type ChEBI dbReference id 2.7.7.72 type EC comment type cofactor cofactor evidence 1 name Mg(2+) dbReference id CHEBI:18420 type ChEBI comment type subunit text evidence 1 Homodimer. comment type miscellaneous text evidence 1 A-single-active-site-specifically-recognizes-both-ATP-and-CTP-and-is-responsible-for-their-addition. comment type similarity text evidence 1 Belongs-to-the-tRNA-nucleotidyltransferase/poly(A)-polymerase-family.-Bacterial-CCA-adding-enzyme-type-3-subfamily. dbReference evidence 1 id 2.7.7.72 type EC dbReference id CP001177 type EMBL property type protein-sequence-ID value ACJ77947.1 property type molecule-type value Genomic_DNA dbReference id WP_000439307.1 type RefSeq property type nucleotide-sequence-ID value NC_011658.1 dbReference id B7HL50 type AlphaFoldDB dbReference id B7HL50 type SMR dbReference id ACJ77947 type EnsemblBacteria property type protein-sequence-ID value ACJ77947 property type gene-ID value BCAH187_A1703 dbReference id bcr:BCAH187_A1703 type KEGG dbReference id CLU_015961_3_0_9 type HOGENOM dbReference id MRAVRFM type OMA dbReference id UP000002214 type Proteomes property type component value Chromosome dbReference id GO:0005524 type GO property type term value F:ATP-binding property type evidence value ECO:0007669 property type project value UniProtKB-UniRule dbReference id GO:0004810 type GO property type term value F:CCA-tRNA-nucleotidyltransferase-activity property type evidence value ECO:0007669 property type project value UniProtKB-UniRule dbReference id GO:0000287 type GO property type term value F:magnesium-ion-binding property type evidence value ECO:0007669 property type project value UniProtKB-UniRule dbReference id GO:0000049 type GO property type term value F:tRNA-binding property type evidence value ECO:0007669 property type project value UniProtKB-UniRule dbReference id GO:0042245 type GO property type term value P:RNA-repair property type evidence value ECO:0007669 property type project value UniProtKB-KW dbReference id GO:0001680 type GO property type term value P:tRNA-3'-terminal-CCA-addition property type evidence value ECO:0007669 property type project value UniProtKB-UniRule dbReference id cd05398 type CDD property type entry-name value NT_ClassII-CCAase property type match-status value 1 dbReference id 1.10.110.30 type Gene3D property type match-status value 1 dbReference id 1.10.246.80 type Gene3D property type match-status value 1 dbReference id 1.20.58.560 type Gene3D property type match-status value 1 dbReference id 3.30.460.10 type Gene3D property type entry-name value Beta-Polymerase,-domain-2 property type match-status value 1 dbReference id MF_01263 type HAMAP property type entry-name value CCA_bact_type3 property type match-status value 1 dbReference id IPR032810 type InterPro property type entry-name value CCA-adding_enz_C dbReference id IPR023068 type InterPro property type entry-name value CCA-adding_enz_firmicutes dbReference id IPR043519 type InterPro property type entry-name value NT_sf dbReference id IPR002646 type InterPro property type entry-name value PolA_pol_head_dom dbReference id IPR032828 type InterPro property type entry-name value PolyA_RNA-bd dbReference id PTHR46173 type PANTHER property type entry-name value CCA-TRNA-NUCLEOTIDYLTRANSFERASE-1,-MITOCHONDRIAL property type match-status value 1 dbReference id PTHR46173:SF1 type PANTHER property type entry-name value CCA-TRNA-NUCLEOTIDYLTRANSFERASE-1,-MITOCHONDRIAL property type match-status value 1 dbReference id PF01743 type Pfam property type entry-name value PolyA_pol property type match-status value 1 dbReference id PF12627 type Pfam property type entry-name value PolyA_pol_RNAbd property type match-status value 1 dbReference id PF13735 type Pfam property type entry-name value tRNA_NucTran2_2 property type match-status value 1 dbReference id SSF81301 type SUPFAM property type entry-name value Nucleotidyltransferase property type match-status value 1 dbReference id SSF81891 type SUPFAM property type entry-name value Poly-A-polymerase-C-terminal-region-like property type match-status value 1 proteinExistence type inferred-from-homology keyword id KW-0067 ATP-binding keyword id KW-0460 Magnesium keyword id KW-0479 Metal-binding keyword id KW-0547 Nucleotide-binding keyword id KW-0548 Nucleotidyltransferase keyword id KW-0692 RNA-repair keyword id KW-0694 RNA-binding keyword id KW-0808 Transferase keyword id KW-0819 tRNA-processing feature description CCA-adding-enzyme id PRO_1000140071 type chain location begin position 1 end position 397 feature evidence 1 type binding-site location position position 26 ligand name ATP dbReference id CHEBI:30616 type ChEBI feature evidence 1 type binding-site location position position 26 ligand name CTP dbReference id CHEBI:37563 type ChEBI feature evidence 1 type binding-site location position position 29 ligand name ATP dbReference id CHEBI:30616 type ChEBI feature evidence 1 type binding-site location position position 29 ligand name CTP dbReference id CHEBI:37563 type ChEBI feature evidence 1 type binding-site location position position 39 ligand name Mg(2+) dbReference id CHEBI:18420 type ChEBI feature evidence 1 type binding-site location position position 41 ligand name Mg(2+) dbReference id CHEBI:18420 type ChEBI feature evidence 1 type binding-site location position position 110 ligand name ATP dbReference id CHEBI:30616 type ChEBI feature evidence 1 type binding-site location position position 110 ligand name CTP dbReference id CHEBI:37563 type ChEBI feature evidence 1 type binding-site location position position 153 ligand name ATP dbReference id CHEBI:30616 type ChEBI feature evidence 1 type binding-site location position position 153 ligand name CTP dbReference id CHEBI:37563 type ChEBI feature evidence 1 type binding-site location position position 156 ligand name ATP dbReference id CHEBI:30616 type ChEBI feature evidence 1 type binding-site location position position 156 ligand name CTP dbReference id CHEBI:37563 type ChEBI feature evidence 1 type binding-site location position position 159 ligand name ATP dbReference id CHEBI:30616 type ChEBI feature evidence 1 type binding-site location position position 159 ligand name CTP dbReference id CHEBI:37563 type ChEBI feature evidence 1 type binding-site location position position 162 ligand name ATP dbReference id CHEBI:30616 type ChEBI feature evidence 1 type binding-site location position position 162 ligand name CTP dbReference id CHEBI:37563 type ChEBI evidence key 1 type ECO:0000255 source dbReference id MF_01263 type HAMAP-Rule sequence checksum 66EB84A1DBC65575 length 397 mass 45914 modified 2009-02-10 version 1 MERFKKASSIIETLKQQGHEAYFVGGSVRDLIIDRPIGDIDIATSALPEEVMAIFPRHVPVGLEHGTVIVVENGEPYEVTTFRTESEYEDFRRPSSVQFVRSLEEDLKRRDFTMNAIAMTEEGKMVDLFAGQEAIQKREIVTVGNAADRFQEDALRMMRGIRFVSTLGFSLETKTKQAIETYGHLLEHIAIERITVEFEKLLTGTYCVKALKELVETKLFSHLPYLQMSEEKLLKATQYKWDSFEADIEAWAFFLYCIGEEHPAVFLRQWKFSNKKIKDIVAVLLTIRKRKEKDWDTVFLYKTGIHIAEMAERVYEAMIESYDHTAVNRVQTLFQALPIKNRQEMNVTGNDLLNWASKKPGPWVAEMIQKIEEAIVQGNVVNEKECIREWLQECNLL 
