LongBarcodeQC

{{ title }}

{{ subtitle }}

Alignment summary

{% for header in headers %} {% endfor %} {% for row in data %} {% for item in row %} {% endfor %} {% endfor %}
{{ header }}
{{ item }}
Total reads: {{ total_reads }}

Read length distributions

{{ read_type }} {{ insertions }} {{ MCS_insertions }}

Z-score distribution and threshold for
calling barcodes

{{ z_plot }}

If the threshold is set too low (too far left within the background alignment z-scores), there will likely be false positive barcode calls. If the threshold is set too high, some true barcodes may be missed. The default value is set based on the absolute value of the lowest background z-score. You may choose a more appropriate threshold based on the distribution of z-scores for your data using the -z option.

Restriction sites

MCS enzyme diagram

MCS diagram
{% for header in headers_enz %} {% endfor %} {% for row in data_enz %} {% for item in row %} {% endfor %} {% endfor %}
{{ header }}
{{ item }}
{% for name in rs_plot_names %} {% endfor %}
{% for pair in rs_plots %}
{{ pair.read | safe }}
{{ pair.mcs | safe }}
{% endfor %}