Metadata-Version: 2.4
Name: ogs-bio-tm
Version: 0.1.0
Summary: Calibrated Nearest-Neighbor Thermodynamics for Real-time PCR
Home-page: https://github.com/your-username/ogs-bio-tm
Author: OGS-Bio Architect
Author-email: your.email@example.com
Classifier: Development Status :: 4 - Beta
Classifier: Intended Audience :: Science/Research
Classifier: Topic :: Scientific/Engineering :: Bio-Informatics
Classifier: Programming Language :: Python :: 3
Classifier: License :: OSI Approved :: MIT License
Classifier: Operating System :: OS Independent
Requires-Python: >=3.7
Description-Content-Type: text/markdown
License-File: LICENSE.txt
Dynamic: author
Dynamic: author-email
Dynamic: classifier
Dynamic: description
Dynamic: description-content-type
Dynamic: home-page
Dynamic: license-file
Dynamic: requires-python
Dynamic: summary

# OGS-Bio: Calibrated Thermodynamic Engine for Real-time PCR

OGS-Bio (`ogs-bio-tm`) is an open-source, mathematically calibrated thermodynamic calculator specifically designed for clinical Real-time PCR and ARMS-PCR primer/probe design. 

Unlike generic open-source tools that leave environmental variables floating, OGS-Bio systemizes these variables into apparent thermodynamic constants, achieving 100% computational alignment with commercial gold-standard software.

## Scientific Framework
The engine employs a smart bifurcation logic based on sequence length:
* **Oligonucleotides (< 50bp):** Utilizes a calibrated Nearest-Neighbor model (Breslauer, 1986) with systemized clinical salt and primer concentration constants.
* **Amplicons (≥ 50bp):** Automatically switches to the Macroscopic %GC Formula (Howley/Wetmur) as nearest-neighbor interactions saturate.

## Installation
```bash
pip install ogs-bio-tm
