BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance.

The NCBI provides a suite of command-line tools to run BLAST called BLAST+. This allows users to perform BLAST searches on their own workstation without size, volume and database restrictions.

BLAST+:	https://blast.ncbi.nlm.nih.gov/doc/blast-help/downloadblastdata.html
Manual:	https://www.ncbi.nlm.nih.gov/books/NBK279690/

Camacho, C., Coulouris, G., Avagyan, V., Ma, N., Papadopoulos, J., Bealer, K., and Madden, T.L. 2009. BLAST+: architecture and applications. BMC Bioinformatics, 10, 421.