
PMID- 39226761
OWN - NLM
STAT- MEDLINE
DCOM- 20240914
LR  - 20240914
IS  - 1873-2690 (Electronic)
IS  - 0981-9428 (Linking)
VI  - 215
DP  - 2024 Oct
TI  - Rice OsCIPK17-OsCBL2/3 module enhances shoot Na(+) exclusion and plant salt 
      tolerance in transgenic Arabidopsis.
PG  - 109034
LID - S0981-9428(24)00702-2 [pii]
LID - 10.1016/j.plaphy.2024.109034 [doi]
AB  - Soil salinity is detrimental to plant growth and remains a major threat to crop 
      productivity of the world. Plants employ various physiological and molecular 
      mechanisms to maintain growth under salt stress. Identification of genes and 
      genetic loci underlying plant salt tolerance holds the key to breeding salt 
      tolerant crops. CIPK-CBL pathways regulate adaptive responses of plants 
      (especially ion transport) to abiotic stresses via fine-tuned Ca(2+) signal 
      transduction. In this study, we showed that over-expression of OsCIPK17 in 
      Arabidopsis enhanced primary root elongation under salt stress, which is in a 
      Ca(2+) dependent manner. Further investigation revealed that, under salt stress, 
      OsCIPK17 transcript level was significantly induced and its protein moved from 
      the cytosol to the tonoplast. Using both Y2H and BiFC, tonoplast-localised OsCBL2 
      and OsCBL3 were shown to interact with OsCIPK17. Interestingly, over-expressing 
      salt-induced OsCBL2 or OsCBL3 in Arabidopsis led to enhanced primary root 
      elongation under salt stress. In this process, OsCIPK17 was shown recruited to 
      the tonoplast (similar to the effect of salt stress). Furthermore, transgenic 
      Arabidopsis lines individually over-expressing OsCIPK17, OsCBL2 and OsCBL3 all 
      demonstrated larger biomass and less Na (+) accumulation in the shoot under salt 
      stress. All data combined suggest that OsCIPK17- OsCBL2/3 module is a major 
      component of shoot Na(+) exclusion and therefore plant salt tolerance, which is 
      through enhanced Na (+) compartmentation into the vacuole in the root. OsCIPK17 
      and OsCBL2/3 are therefore potential genetic targets that can be used for 
      delivering salt tolerant rice cultivars.
CI  - Copyright (c) 2024. Published by Elsevier Masson SAS.
FAU - Qin, Xin
AU  - Qin X
AD  - Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, 
      School of Life Sciences, Lanzhou University, Lanzhou, 730000, China; Key 
      Laboratory of Gene Editing for Breeding, Gansu Province, Lanzhou 730000, China.
FAU - Zhang, Xiaohua
AU  - Zhang X
AD  - Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, 
      School of Life Sciences, Lanzhou University, Lanzhou, 730000, China; Key 
      Laboratory of Gene Editing for Breeding, Gansu Province, Lanzhou 730000, China.
FAU - Ma, Chenyujie
AU  - Ma C
AD  - Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, 
      School of Life Sciences, Lanzhou University, Lanzhou, 730000, China; Key 
      Laboratory of Gene Editing for Breeding, Gansu Province, Lanzhou 730000, China.
FAU - Yang, Xue
AU  - Yang X
AD  - Jiayuguan Ecological and Environmental Monitoring Center, Jiayuguan 735100, 
      China.
FAU - Hu, Yibo
AU  - Hu Y
AD  - Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, 
      School of Life Sciences, Lanzhou University, Lanzhou, 730000, China; Key 
      Laboratory of Gene Editing for Breeding, Gansu Province, Lanzhou 730000, China.
FAU - Liu, Yuan
AU  - Liu Y
AD  - Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, 
      School of Life Sciences, Lanzhou University, Lanzhou, 730000, China; Key 
      Laboratory of Gene Editing for Breeding, Gansu Province, Lanzhou 730000, China.
FAU - Hu, Yunfei
AU  - Hu Y
AD  - Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, 
      School of Life Sciences, Lanzhou University, Lanzhou, 730000, China; Key 
      Laboratory of Gene Editing for Breeding, Gansu Province, Lanzhou 730000, China.
FAU - Wang, Dan
AU  - Wang D
AD  - Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, 
      School of Life Sciences, Lanzhou University, Lanzhou, 730000, China; Key 
      Laboratory of Gene Editing for Breeding, Gansu Province, Lanzhou 730000, China.
FAU - Xiaodong Lv
AU  - Xiaodong Lv
AD  - Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, 
      School of Life Sciences, Lanzhou University, Lanzhou, 730000, China; Key 
      Laboratory of Gene Editing for Breeding, Gansu Province, Lanzhou 730000, China.
FAU - Wang, Chao
AU  - Wang C
AD  - College of Life Sciences, Shaoxing University, Shaoxing 312000, China.
FAU - Shou, Jianxin
AU  - Shou J
AD  - Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, 
      School of Life Sciences, Lanzhou University, Lanzhou, 730000, China; College of 
      Life Sciences, Shaoxing University, Shaoxing 312000, China. Electronic address: 
      jianxinshou@usx.edu.cn.
FAU - Li, Bo
AU  - Li B
AD  - Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, 
      School of Life Sciences, Lanzhou University, Lanzhou, 730000, China; Key 
      Laboratory of Gene Editing for Breeding, Gansu Province, Lanzhou 730000, China. 
      Electronic address: li_bo@lzu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20240815
PL  - France
TA  - Plant Physiol Biochem
JT  - Plant physiology and biochemistry : PPB
JID - 9882449
RN  - 0 (Plant Proteins)
RN  - 9NEZ333N27 (Sodium)
SB  - IM
MH  - *Arabidopsis/genetics/metabolism
MH  - *Plants, Genetically Modified
MH  - *Oryza/genetics/metabolism
MH  - *Salt Tolerance/genetics
MH  - *Plant Shoots/genetics/metabolism
MH  - *Plant Proteins/genetics/metabolism
MH  - *Sodium/metabolism
MH  - Gene Expression Regulation, Plant
MH  - Plant Roots/genetics/metabolism
OTO - NOTNLM
OT  - Arabidopsis
OT  - Na(+) exclusion
OT  - Salinity tolerance
OT  - Vacuolar compartmentation
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2024/09/04 01:01
MHDA- 2024/09/14 10:46
CRDT- 2024/09/03 18:06
PHST- 2023/12/28 00:00 [received]
PHST- 2024/07/30 00:00 [revised]
PHST- 2024/08/08 00:00 [accepted]
PHST- 2024/09/14 10:46 [medline]
PHST- 2024/09/04 01:01 [pubmed]
PHST- 2024/09/03 18:06 [entrez]
AID - S0981-9428(24)00702-2 [pii]
AID - 10.1016/j.plaphy.2024.109034 [doi]
PST - ppublish
SO  - Plant Physiol Biochem. 2024 Oct;215:109034. doi: 10.1016/j.plaphy.2024.109034. 
      Epub 2024 Aug 15.
