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Summary of the required and optional arguments for the V(D)J annotation tool.
Argument
Description
-r, --receptor-typeType of receptor to analyze. Choices:
IG (immunoglobulin) or TR (T-cell receptor). Required when using --default.
-i, --input
or-a, --assembly
--input: directory containing extracted sequence regions (already isolated from an assembly)
--assembly: directory containing a complete genome assembly
-l, --libraryPath to the FASTA library file containing reference V(D)J segment sequences.
-f, --flanking-genes
Comma-separated list of flanking genes in JSON format. For a single flanking gene, use
"-" as a placeholder.
-s, --speciesScientific species name (e.g., Homo sapiens).
-M, --metadataPath to the metadata file (
.xlsx). Download example template here.
-o, --outputOutput directory for the results (default:
annotation_results).
-m, --mapping-toolMapping tool(s) to use:
minimap2, bowtie, bowtie2 (default: all).
-t, --threadsNumber of threads for parallel processing (default: 8).
--defaultUse default settings. Cannot be used together with
--flanking-genes.
-S, --scaffoldingPath to reference genome (FASTA). Only supported for phased assembly files.
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