RosettaX documentation#
RosettaX calibrates exported FCS data for fluorescence and scattering workflows and then reapplies saved calibrations to experimental files. This reference focuses on practical workflow guidance and the modeling details behind each output.
Choose your path#
First run and setup
Start here if you are configuring RosettaX for the first time or walking through the interface.
Calibration workflows
Follow the complete workflow sequence for fluorescence, scattering, peak identification, and apply-calibration steps.
Modeling and internals
Use these pages when you want the theory, assumptions, and implementation references behind the calculations and generated reports.
Recommended reading order#
If you want the technical picture first, read these pages in order:
Full documentation map#
Under the hood
- Implementation model
- What kind of flow-cytometry system is modeled
- How material refractive indices are computed
- Regression models used by RosettaX
- Why the saved record matters
- Size parameter and refractive index contrast
- Angular collection
- Calibration curves and monotonicity
- Instrument response
- Noise and detection limits
- Triggering and selection bias
- Pulse features
- Fitting calibration relations
- Interpolation and extrapolation
- Calibration export
- Runtime profiles
- Local execution and reproducibility
- Limitations
- Best practices
- Use appropriate reference materials
- Inspect the data before fitting
- Avoid unsupported extrapolation
- Keep acquisition settings consistent
- Review monotonicity for scattering calibration
- Preserve calibration metadata
- Workflow guides
- Calibration file format
- Reports and export provenance
Getting started and debugging