Quick start

This page is the fastest way to decide where to begin in RosettaX.

Choose the right workflow

Use Fluorescence when you are mapping measured detector values to known fluorescence reference values such as MESF.

Use Scattering when you are fitting measured scatter peaks against modeled or tabulated scattering standards.

Use Apply calibration when you already trust the saved calibration JSON and want to export calibrated FCS files.

Recommended order of work

1. Upload the calibration bead or standard file.

2. Inspect the selected detector and the visible peaks before fitting.

3. Review the calibration table carefully.

4. Create the calibration and inspect the fit.

5. Save the calibration JSON.

6. Reuse the calibration in the apply workflow and keep the generated PDF report.

What to inspect before saving

Before saving a calibration, confirm all of the following:

• the source channel is the detector you actually intend to calibrate

• the measured peaks correspond to the expected bead populations

• the calibration table pairs the right measured values with the right references

• the fitted relation is scientifically plausible, not just numerically tidy

• the metadata is specific enough that another person could interpret the JSON later

What to inspect before applying

Before exporting calibrated files, confirm all of the following:

• the selected calibration matches the intended detector and quantity

• the uploaded experimental files belong to the same interpretation context as the saved calibration

• any scattering target-model settings are still appropriate

• the exported channels and extra columns are intentional

Next pages

Continue with Interface guide for a page-by-page guide, then read the workflow chapter that matches your task in Workflow guides.