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96-well plate RNA barcode demultiplexing & clustering
GitHub
Try sample data
Setup checklist
1
Upload your sequencing file (FASTQ or plain-text reads).
Each line containing a read is scanned. FASTQ headers/quality lines are ignored automatically.
2
Paste your row & column barcodes (one per line) — these tag each well.
3
Set the 5′ and 3′ flanking sequences that bracket the variable RNA barcode.
4
Hit
Run analysis
. Results appear in real-time on the right.
Input
Sequencing data (.fastq or .txt)
Click or drop file here
Row barcodes (one per line)
Column barcodes (one per line)
5′ flank
3′ flank
Expected barcode length (nt)
Edit tolerance (Levenshtein)
Use 20-nt left-flank offset (take last N nt between flanks)
Plate IDs (optional, one per line)
Run analysis
Try with sample data
Results
idle
Hover or click a well to see its top barcodes.
Download summary.txt
Download results.pdf