Analysis results - {{ title }}

Fitting results

Bead centroid's coordinates: {{ bead._centroid if bead._centroid is not none else "N/A" }}

Localisation

Localisation.png

X profile fit

fit_curve_1D_X.png

Y profile fit

fit_curve_1D_Y.png

Z profile fit

fit_curve_1D_Z.png

Z view

XY_view.png

Y view

XZ_view.png

X view

YZ_view.png

Tilt XZ

Tilt XZ not available

Tilt ZY

Tilt ZY not available

Resolution & Fitting Quality

Parameter X-axis Y-axis Z-axis
Theoretical resolution (µm) {{ "%.4f"|format(theoretical_resolution[2] if theoretical_resolution[2] is not none else 0) }} {{ "%.4f"|format(theoretical_resolution[1] if theoretical_resolution[1] is not none else 0) }} {{ "%.4f"|format(theoretical_resolution[0] if theoretical_resolution[0] is not none else 0) }}
Sampling distance (µm) {{ "%.4f"|format(sampling_distance[2] if sampling_distance[2] is not none else 0) }} {{ "%.4f"|format(sampling_distance[1] if sampling_distance[1] is not none else 0) }} {{ "%.4f"|format(sampling_distance[0] if sampling_distance[0] is not none else 0) }}
Full width at half maximum (µm) {{ "%.4f"|format(bead._fitTool.fwhms[2] if bead._fitTool.fwhms[2] is not none else 0) }} {{ "%.4f"|format(bead._fitTool.fwhms[1] if bead._fitTool.fwhms[1] is not none else 0) }} {{ "%.4f"|format(bead._fitTool.fwhms[0] if bead._fitTool.fwhms[0] is not none else 0) }}
Uncertainty {{ "%.4f"|format(bead._fitTool.uncertainties[2][3] if bead._fitTool.uncertainties[2] is not none else 0) }} {{ "%.4f"|format(bead._fitTool.uncertainties[1][3] if bead._fitTool.uncertainties[1] is not none else 0) }} {{ "%.4f"|format(bead._fitTool.uncertainties[0][3] if bead._fitTool.uncertainties[0] is not none else 0) }}
Determination coefficient (R²) {{ "%.4f"|format(bead._fitTool.determinations[2] if bead._fitTool.determinations[2] is not none else 0) }} {{ "%.4f"|format(bead._fitTool.determinations[1] if bead._fitTool.determinations[1] is not none else 0) }} {{ "%.4f"|format(bead._fitTool.determinations[0] if bead._fitTool.determinations[0] is not none else 0) }}

Metrics

Standard metrics

Signal to Background: {% set sbr_help %} SBR > 10 : the signal is much higher than the background, the image quality is good.
SBR > 5 and < 10 : the signal is higher than the background, the image quality is fair.
SBR < 5 : the signal is not much higher than the background, the image quality may be limited.

{% endset %} {% set sbr = bead._metricTool._SBR if bead._metricTool._SBR is not none else 0 %} {% if sbr > 10 %} {{ sbr_help }}Good : Signal to background ratio is high, the image quality is good. {% elif sbr > 5 %} {{ sbr_help }}Warning : Signal to background is moderate, the analysis may have some limitations {% else %} {{ sbr_help }}Bad : Signal to background is low, the analysis may be unreliable{% endif %} {{ sbr|round(4) if bead._metricTool._SBR is not none else "N/A" }}

Contrast: {% set contrast_help %} Contrast > 10 : the signal is much higher than the background, the image quality is good.
Contrast > 5 and < 10 : the signal is higher than the background, the image quality is fair.
Contrast < 5 : the signal is not much higher than the background, the image quality may be limited.

{% endset %} {% set contrast = bead._fitTool.contrast if bead._fitTool.contrast is not none else 0 %} {% if contrast > 10 %} {{ contrast_help }}Good : Contrast is high, the image quality is good. {% elif contrast > 5 %} {{ contrast_help }}Warning : Contrast is moderate, the analysis may have some limitations {% else %} {{ contrast_help }}Bad : Contrast is low, the analysis may be unreliable{% endif %} {{ "%.4f"|format(bead._fitTool.contrast if bead._fitTool.contrast is not none else 0) }}

EllipsRatio: {% set ellipsRatio_help %} EllipsRatio > 0.9 and < 1.1 : Main axis are equivalent, the bead is circular.
EllipsRatio > 0.8 and < 1.2 : Main axis are somewhat different, the bead is somewhat elongated.
EllipsRatio < 0.8 or > 1.2 : Main axis are very different, the bead is very elongated.

{% endset %} {% set ellipsRatio = bead._metricTool._ellipsRatio if bead._metricTool._ellipsRatio is not none else 0 %} {% if ellipsRatio > 0.9 and ellipsRatio < 1.1 %} {{ ellipsRatio_help }}Good : Main axis are equivalent, the bead is circular. {% elif ellipsRatio > 0.8 and ellipsRatio < 1.2 %} {{ ellipsRatio_help }}Warning : Main axis are somewhat different, the bead is somewhat elongated. {% else %} {{ ellipsRatio_help }}Bad : Main axis are very different, the bead is very elongated.{% endif %} {{ "%.4f"|format(bead._metricTool._ellipsRatio if bead._metricTool._ellipsRatio is not none else 0) }}

Orientation: {{ "%.4f"|format(bead._metricTool._orientation if bead._metricTool._orientation is not none else 0) }}

Lateral asymmetry ratio (LAR): {% set lar_help %} LAR > 0.9 : LAR is high, the bead is symmetric.
LAR > 0.8 and < 0.9 : LAR is moderate, the bead is somewhat elongated.
LAR < 0.8 : LAR is low, the bead is very elongated.

{% endset %} {% set lar = bead._metricTool._LAR if bead._metricTool._LAR is not none else 0 %} {% if lar > 0.9 %} {{ lar_help }}Good : LAR is high, the bead is symmetric. {% elif lar > 0.8 %} {{ lar_help }}Warning : The bead is somewhat elongated. {% else %} {{ lar_help }}Bad : The bead is very elongated.{% endif %} {{ lar|round(4) if bead._metricTool._LAR is not none else "N/A" }}

Sphericity: {% set sphericity_help %} Sphericity > 0.75 : Sphericity is high, the image quality is good.
Sphericity > 0.65 and < 0.75 : Sphericity is moderate, the bead is somewhat elongated.
Sphericity < 0.65 : Sphericity is low, the bead is very elongated.

{% endset %} {% set sphericity = bead._metricTool._sphericity if bead._metricTool._sphericity is not none else 0 %} {% if sphericity > 0.75 %} {{ sphericity_help }}Good : Sphericity is high, the image quality is good. {% elif sphericity > 0.65 %} {{ sphericity_help }}Warning : Sphericity is moderate, the bead is somewhat elongated. {% else %} {{ sphericity_help }}Bad : Sphericity is low, the bead is very elongated.{% endif %} {{ sphericity|round(4) if bead._metricTool._sphericity is not none else "N/A" }}

Comatic Aberration Metrics

Comaticity: {% set comaticity_help %} Comaticity < 0.1 : Comaticity is low, the bead is not comatic.
Comaticity < 0.2 : Comaticity is moderate, the bead is somewhat comatic.
Comaticity >= 0.2 : Comaticity is high, the bead is highly comatic.

{% endset %} {% set comaticity = bead._metricTool._comaticity if bead._metricTool._comaticity is not none else 0 %} {% if comaticity < 0.1 %} {{ comaticity_help }}Good : Comaticity is low, the image quality is good. {% elif comaticity < 0.2 %} {{ comaticity_help }}Warning : Comaticity is moderate, you may check if the objective lens is tipped and translated with respect to the optic axis. {% else %} {{ comaticity_help }}Bad : Comaticity is high, you have to check if the objective lens is tipped and translated with respect to the optic axis.{% endif %} {{ comaticity|round(4) if bead._metricTool._comaticity is not none else "N/A" }}

Skeleton to extremities: {% set skeletonToExtremities_help %} Skeleton to extremities ratio > 0.9 and < 1.1 : Skeleton to extremities ratio is high, the bead is not comatic.
Skeleton to extremities ratio > 0.8 and < 1.2 : Skeleton to extremities ratio is moderate, the bead is somewhat comatic.
Skeleton to extremities ratio < 0.8 or > 1.2 : Skeleton to extremities ratio is far from 1, the bead is highly comatic.

{% endset %} {% set skeletonToExtremities = bead._metricTool._skeleton2Extremities if bead._metricTool._skeleton2Extremities is not none else 0 %} {% if skeletonToExtremities > 0.9 and skeletonToExtremities < 1.1 %} {{skeletonToExtremities_help}}Good : Skeleton to extremities ratio is high, the image quality is good. {% elif skeletonToExtremities > 0.8 and skeletonToExtremities < 1.2 %} {{skeletonToExtremities_help}}Warning : Skeleton to extremities ratio is moderate, you may check if the objective lens is tipped and translated with respect to the optic axis. {% else %} {{skeletonToExtremities_help}}Bad : Skeleton to extremities ratio is far from 1, you have to check if the objective lens is tipped and translated with respect to the optic axis.{% endif %} {{ skeletonToExtremities|round(4) if bead._metricTool._skeleton2Extremities is not none else "N/A" }}

Concavity: {% set concavity_help %} Concavity < 0.15 : Concavity is low, the bead is not comatic.
Concavity < 0.25 : Concavity is moderate, the bead is somewhat comatic.
Concavity >= 0.25 : Concavity is high, the bead is highly comatic.

{% endset %} {% set concavity = bead._metricTool.meshBuilder._concavity if bead._metricTool.meshBuilder._concavity is not none else 0 %} {% if concavity < 0.15 %} {{concavity_help}}Good : Concavity is low, the image quality is good. {% elif concavity < 0.25 %} {{concavity_help}}Warning : Concavity is moderate, you may check if the objective lens is tipped and translated with respect to the optic axis. {% else %} {{concavity_help}}Bad : Concavity is high, you have to check if the objective lens is tipped and translated with respect to the optic axis.{% endif %} {{ concavity|round(4) if bead._metricTool.meshBuilder._concavity is not none else "N/A" }}

Minimum curvature radius centroids path: {% set minCurvatureRadiusCentroidsPath_help %} Minimum curvature radius < 0.1 : Minimum curvature radius is high, the bead is not comatic.
Minimum curvature radius < 0.2 and >= 0.1 : Minimum curvature radius is moderate, the bead is somewhat comatic.
Minimum curvature radius >= 0.2 : Minimum curvature radius is low, the bead is highly comatic.

{% endset %} {% set minCurvatureRadiusCentroidsPath = bead._metricTool._RMin if bead._metricTool._RMin is not none else 0 %} {% if minCurvatureRadiusCentroidsPath < 0.1 %} {{minCurvatureRadiusCentroidsPath_help}}Good : Minimum curvature radius is high, the image quality is good. {% elif minCurvatureRadiusCentroidsPath < 0.2 %} {{minCurvatureRadiusCentroidsPath_help}}Warning : Minimum curvature radius is moderate, you may check if the objective lens is tipped and translated with respect to the optic axis. {% else %} {{minCurvatureRadiusCentroidsPath_help}}Bad : Minimum curvature radius is low, you have to check if the objective lens is tipped and translated with respect to the optic axis.{% endif %} {{ minCurvatureRadiusCentroidsPath|round(4) if bead._metricTool._RMin is not none else "N/A" }}

Astigmatism Metrics

Astigmatism: {% set astigmatism_help %} Astigmatism < 0.1 and > -0.1 : Astigmatism is low, the bead is not astigmatic.
Astigmatism < 0.2 and > -0.2 : Astigmatism is moderate, the bead is somewhat astigmatic.
Astigmatism >= 0.2 or <= -0.2 : Astigmatism is high, the bead is highly astigmatic.

{% endset %} {% set astigmatism = bead._metricTool._astigmatism if bead._metricTool._astigmatism is not none else 0 %} {% if astigmatism < 0.1 and astigmatism > -0.1 %} {{astigmatism_help}}Good : Astigmatism is low, the image quality is good. {% elif astigmatism < 0.2 and astigmatism > -0.2 %} {{astigmatism_help}}Warning : Astigmatism is moderate, you may check if the focus is properly adjusted and the objective lens quality. {% else %} {{astigmatism_help}}Bad : Astigmatism is high, you have to check if the focus is properly adjusted and the objective lens quality.{% endif %} {{ astigmatism|round(4) if bead._metricTool._astigmatism is not none else "N/A" }}

{% if bead._metricTool._commentary is not none %}

{{ bead._metricTool._commentary }}

{% endif %}

Spherical Aberration Metrics

Spherical aberration: {% set sa_help %} Spherical aberration < 0.05 : Spherical aberration is low, the bead is not spherical.
Spherical aberration < 0.1 : Spherical aberration is moderate, the bead is somewhat spherical.
Spherical aberration >= 0.2 : Spherical aberration is high, the bead is highly spherical.

{% endset %} {% set sa = bead._metricTool._sphericalAberration if bead._metricTool._sphericalAberration is not none else 0 %} {% if sa < 0.05 %} {{sa_help}}Good : Spherical aberration is low, the image quality is good. {% elif sa < 0.1 %} {{sa_help}}Warning : Spherical aberration is moderate, you may check if the sample is properly mounted and the coverslip thickness is correct. {% else %} {{sa_help}}Bad : Spherical aberration is high, you have to check if the sample is properly mounted and the coverslip thickness is correct.{% endif %} {{ sa|round(4) if bead._metricTool._sphericalAberration is not none else "N/A" }}