Bead centroid's coordinates: {{ bead._centroid if bead._centroid is not none else "N/A" }}
| Parameter | X-axis | Y-axis | Z-axis |
|---|---|---|---|
| Theoretical resolution (µm) | {{ "%.4f"|format(theoretical_resolution[2] if theoretical_resolution[2] is not none else 0) }} | {{ "%.4f"|format(theoretical_resolution[1] if theoretical_resolution[1] is not none else 0) }} | {{ "%.4f"|format(theoretical_resolution[0] if theoretical_resolution[0] is not none else 0) }} |
| Sampling distance (µm) | {{ "%.4f"|format(sampling_distance[2] if sampling_distance[2] is not none else 0) }} | {{ "%.4f"|format(sampling_distance[1] if sampling_distance[1] is not none else 0) }} | {{ "%.4f"|format(sampling_distance[0] if sampling_distance[0] is not none else 0) }} |
| Full width at half maximum (µm) | {{ "%.4f"|format(bead._fitTool.fwhms[2] if bead._fitTool.fwhms[2] is not none else 0) }} | {{ "%.4f"|format(bead._fitTool.fwhms[1] if bead._fitTool.fwhms[1] is not none else 0) }} | {{ "%.4f"|format(bead._fitTool.fwhms[0] if bead._fitTool.fwhms[0] is not none else 0) }} |
| Uncertainty | {{ "%.4f"|format(bead._fitTool.uncertainties[2][3] if bead._fitTool.uncertainties[2] is not none else 0) }} | {{ "%.4f"|format(bead._fitTool.uncertainties[1][3] if bead._fitTool.uncertainties[1] is not none else 0) }} | {{ "%.4f"|format(bead._fitTool.uncertainties[0][3] if bead._fitTool.uncertainties[0] is not none else 0) }} |
| Determination coefficient (R²) | {{ "%.4f"|format(bead._fitTool.determinations[2] if bead._fitTool.determinations[2] is not none else 0) }} | {{ "%.4f"|format(bead._fitTool.determinations[1] if bead._fitTool.determinations[1] is not none else 0) }} | {{ "%.4f"|format(bead._fitTool.determinations[0] if bead._fitTool.determinations[0] is not none else 0) }} |
Signal to Background:
{% set sbr_help %}
SBR > 10 : the signal is much higher than the background, the image quality is good.
SBR > 5 and < 10 : the signal is higher than the background, the image quality is fair.
SBR < 5 : the signal is not much higher than the background, the image quality may be limited.
{% endset %}
{% set sbr = bead._metricTool._SBR if bead._metricTool._SBR is not none else 0 %}
{% if sbr > 10 %} {{ sbr_help }}Good : Signal to background ratio is high, the image quality is good.
{% elif sbr > 5 %} {{ sbr_help }}Warning : Signal to background is moderate, the analysis may have some limitations
{% else %} {{ sbr_help }}Bad : Signal to background is low, the analysis may be unreliable{% endif %}
{{ sbr|round(4) if bead._metricTool._SBR is not none else "N/A" }}
Contrast:
{% set contrast_help %}
Contrast > 10 : the signal is much higher than the background, the image quality is good.
Contrast > 5 and < 10 : the signal is higher than the background, the image quality is fair.
Contrast < 5 : the signal is not much higher than the background, the image quality may be limited.
{% endset %}
{% set contrast = bead._fitTool.contrast if bead._fitTool.contrast is not none else 0 %}
{% if contrast > 10 %} {{ contrast_help }}Good : Contrast is high, the image quality is good.
{% elif contrast > 5 %} {{ contrast_help }}Warning : Contrast is moderate, the analysis may have some limitations
{% else %} {{ contrast_help }}Bad : Contrast is low, the analysis may be unreliable{% endif %}
{{ "%.4f"|format(bead._fitTool.contrast if bead._fitTool.contrast is not none else 0) }}
EllipsRatio:
{% set ellipsRatio_help %}
EllipsRatio > 0.9 and < 1.1 : Main axis are equivalent, the bead is circular.
EllipsRatio > 0.8 and < 1.2 : Main axis are somewhat different, the bead is somewhat elongated.
EllipsRatio < 0.8 or > 1.2 : Main axis are very different, the bead is very elongated.
{% endset %}
{% set ellipsRatio = bead._metricTool._ellipsRatio if bead._metricTool._ellipsRatio is not none else 0 %}
{% if ellipsRatio > 0.9 and ellipsRatio < 1.1 %} {{ ellipsRatio_help }}Good : Main axis are equivalent, the bead is circular.
{% elif ellipsRatio > 0.8 and ellipsRatio < 1.2 %} {{ ellipsRatio_help }}Warning : Main axis are somewhat different, the bead is somewhat elongated.
{% else %} {{ ellipsRatio_help }}Bad : Main axis are very different, the bead is very elongated.{% endif %}
{{ "%.4f"|format(bead._metricTool._ellipsRatio if bead._metricTool._ellipsRatio is not none else 0) }}
Orientation: {{ "%.4f"|format(bead._metricTool._orientation if bead._metricTool._orientation is not none else 0) }}
Lateral asymmetry ratio (LAR):
{% set lar_help %}
LAR > 0.9 : LAR is high, the bead is symmetric.
LAR > 0.8 and < 0.9 : LAR is moderate, the bead is somewhat elongated.
LAR < 0.8 : LAR is low, the bead is very elongated.
{% endset %}
{% set lar = bead._metricTool._LAR if bead._metricTool._LAR is not none else 0 %}
{% if lar > 0.9 %} {{ lar_help }}Good : LAR is high, the bead is symmetric.
{% elif lar > 0.8 %} {{ lar_help }}Warning : The bead is somewhat elongated.
{% else %} {{ lar_help }}Bad : The bead is very elongated.{% endif %}
{{ lar|round(4) if bead._metricTool._LAR is not none else "N/A" }}
Sphericity:
{% set sphericity_help %}
Sphericity > 0.75 : Sphericity is high, the image quality is good.
Sphericity > 0.65 and < 0.75 : Sphericity is moderate, the bead is somewhat elongated.
Sphericity < 0.65 : Sphericity is low, the bead is very elongated.
{% endset %}
{% set sphericity = bead._metricTool._sphericity if bead._metricTool._sphericity is not none else 0 %}
{% if sphericity > 0.75 %} {{ sphericity_help }}Good : Sphericity is high, the image quality is good.
{% elif sphericity > 0.65 %} {{ sphericity_help }}Warning : Sphericity is moderate, the bead is somewhat elongated.
{% else %} {{ sphericity_help }}Bad : Sphericity is low, the bead is very elongated.{% endif %}
{{ sphericity|round(4) if bead._metricTool._sphericity is not none else "N/A" }}
Comaticity:
{% set comaticity_help %}
Comaticity < 0.1 : Comaticity is low, the bead is not comatic.
Comaticity < 0.2 : Comaticity is moderate, the bead is somewhat comatic.
Comaticity >= 0.2 : Comaticity is high, the bead is highly comatic.
{% endset %}
{% set comaticity = bead._metricTool._comaticity if bead._metricTool._comaticity is not none else 0 %}
{% if comaticity < 0.1 %} {{ comaticity_help }}Good : Comaticity is low, the image quality is good.
{% elif comaticity < 0.2 %} {{ comaticity_help }}Warning : Comaticity is moderate, you may check if the objective lens is tipped and translated with respect to the optic axis.
{% else %} {{ comaticity_help }}Bad : Comaticity is high, you have to check if the objective lens is tipped and translated with respect to the optic axis.{% endif %}
{{ comaticity|round(4) if bead._metricTool._comaticity is not none else "N/A" }}
Skeleton to extremities:
{% set skeletonToExtremities_help %}
Skeleton to extremities ratio > 0.9 and < 1.1 : Skeleton to extremities ratio is high, the bead is not comatic.
Skeleton to extremities ratio > 0.8 and < 1.2 : Skeleton to extremities ratio is moderate, the bead is somewhat comatic.
Skeleton to extremities ratio < 0.8 or > 1.2 : Skeleton to extremities ratio is far from 1, the bead is highly comatic.
{% endset %}
{% set skeletonToExtremities = bead._metricTool._skeleton2Extremities if bead._metricTool._skeleton2Extremities is not none else 0 %}
{% if skeletonToExtremities > 0.9 and skeletonToExtremities < 1.1 %} {{skeletonToExtremities_help}}Good : Skeleton to extremities ratio is high, the image quality is good.
{% elif skeletonToExtremities > 0.8 and skeletonToExtremities < 1.2 %} {{skeletonToExtremities_help}}Warning : Skeleton to extremities ratio is moderate, you may check if the objective lens is tipped and translated with respect to the optic axis.
{% else %} {{skeletonToExtremities_help}}Bad : Skeleton to extremities ratio is far from 1, you have to check if the objective lens is tipped and translated with respect to the optic axis.{% endif %}
{{ skeletonToExtremities|round(4) if bead._metricTool._skeleton2Extremities is not none else "N/A" }}
Concavity:
{% set concavity_help %}
Concavity < 0.15 : Concavity is low, the bead is not comatic.
Concavity < 0.25 : Concavity is moderate, the bead is somewhat comatic.
Concavity >= 0.25 : Concavity is high, the bead is highly comatic.
{% endset %}
{% set concavity = bead._metricTool.meshBuilder._concavity if bead._metricTool.meshBuilder._concavity is not none else 0 %}
{% if concavity < 0.15 %} {{concavity_help}}Good : Concavity is low, the image quality is good.
{% elif concavity < 0.25 %} {{concavity_help}}Warning : Concavity is moderate, you may check if the objective lens is tipped and translated with respect to the optic axis.
{% else %} {{concavity_help}}Bad : Concavity is high, you have to check if the objective lens is tipped and translated with respect to the optic axis.{% endif %}
{{ concavity|round(4) if bead._metricTool.meshBuilder._concavity is not none else "N/A" }}
Minimum curvature radius centroids path:
{% set minCurvatureRadiusCentroidsPath_help %}
Minimum curvature radius < 0.1 : Minimum curvature radius is high, the bead is not comatic.
Minimum curvature radius < 0.2 and >= 0.1 : Minimum curvature radius is moderate, the bead is somewhat comatic.
Minimum curvature radius >= 0.2 : Minimum curvature radius is low, the bead is highly comatic.
{% endset %}
{% set minCurvatureRadiusCentroidsPath = bead._metricTool._RMin if bead._metricTool._RMin is not none else 0 %}
{% if minCurvatureRadiusCentroidsPath < 0.1 %} {{minCurvatureRadiusCentroidsPath_help}}Good : Minimum curvature radius is high, the image quality is good.
{% elif minCurvatureRadiusCentroidsPath < 0.2 %} {{minCurvatureRadiusCentroidsPath_help}}Warning : Minimum curvature radius is moderate, you may check if the objective lens is tipped and translated with respect to the optic axis.
{% else %} {{minCurvatureRadiusCentroidsPath_help}}Bad : Minimum curvature radius is low, you have to check if the objective lens is tipped and translated with respect to the optic axis.{% endif %}
{{ minCurvatureRadiusCentroidsPath|round(4) if bead._metricTool._RMin is not none else "N/A" }}
Astigmatism:
{% set astigmatism_help %}
Astigmatism < 0.1 and > -0.1 : Astigmatism is low, the bead is not astigmatic.
Astigmatism < 0.2 and > -0.2 : Astigmatism is moderate, the bead is somewhat astigmatic.
Astigmatism >= 0.2 or <= -0.2 : Astigmatism is high, the bead is highly astigmatic.
{% endset %}
{% set astigmatism = bead._metricTool._astigmatism if bead._metricTool._astigmatism is not none else 0 %}
{% if astigmatism < 0.1 and astigmatism > -0.1 %} {{astigmatism_help}}Good : Astigmatism is low, the image quality is good.
{% elif astigmatism < 0.2 and astigmatism > -0.2 %} {{astigmatism_help}}Warning : Astigmatism is moderate, you may check if the focus is properly adjusted and the objective lens quality.
{% else %} {{astigmatism_help}}Bad : Astigmatism is high, you have to check if the focus is properly adjusted and the objective lens quality.{% endif %}
{{ astigmatism|round(4) if bead._metricTool._astigmatism is not none else "N/A" }}
{{ bead._metricTool._commentary }}
{% endif %}Spherical aberration:
{% set sa_help %}
Spherical aberration < 0.05 : Spherical aberration is low, the bead is not spherical.
Spherical aberration < 0.1 : Spherical aberration is moderate, the bead is somewhat spherical.
Spherical aberration >= 0.2 : Spherical aberration is high, the bead is highly spherical.
{% endset %}
{% set sa = bead._metricTool._sphericalAberration if bead._metricTool._sphericalAberration is not none else 0 %}
{% if sa < 0.05 %} {{sa_help}}Good : Spherical aberration is low, the image quality is good.
{% elif sa < 0.1 %} {{sa_help}}Warning : Spherical aberration is moderate, you may check if the sample is properly mounted and the coverslip thickness is correct.
{% else %} {{sa_help}}Bad : Spherical aberration is high, you have to check if the sample is properly mounted and the coverslip thickness is correct.{% endif %}
{{ sa|round(4) if bead._metricTool._sphericalAberration is not none else "N/A" }}