# Third-party data bundled with this package

## Overhang-ligation matrices

`src/ipd_assembly/data/*.csv` (BsmBI-v2, Esp3I, BbsI-HF and SapI) are derived from the
supplementary tables of:

> Pryor JM, Potapov V, Kucera RB, Bilotti K, Cantor EJ, Lohman GJS (2020)
> Enabling one-pot Golden Gate assemblies of unprecedented complexity using
> data-optimized assembly design. *PLoS ONE* 15(9): e0238592.
> https://doi.org/10.1371/journal.pone.0238592

Used under the **Creative Commons Attribution 4.0 International (CC BY 4.0)** licence:
https://creativecommons.org/licenses/by/4.0/

Conditions are T4 ligase, 30 cycles of 5 min at each temperature. `src/ipd_assembly/data/SOURCES.md`
records which supplementary table each file came from, the read counts, and the small differences
between the BsaI-HFv2 table shipped here and the paper's table S1.

## Amplification primer tables

`src/ipd_assembly/data/primers/*.csv` are the Institute for Protein Design's in-house
amplification primer sets, distributed with this package under the same MIT licence as the code.
