h1: variant-to-construct
section insert
h2: insert
table: Part | Role | Bases | Residues | Stop codon removed
section recode
h2: recode
table: Sequence | Constraints met | GC fraction | Lowest window GC (fraction) | Highest window GC (fraction) | Motif matches | GC violations
section build
h2: build
note: 3 vector features are not annotated on the product because they are outside the retained backbone.
table: Primer | Sequence | Annealing footprint | Footprint Tm (°C)
