The recordings of the file, left. Click one to open it; drag the column's right edge to resize it. Below: the unit's own metadata (all… lists every attribute).
Wheel zooms about the cursor, drag a box zooms to it, double-click shows the whole frame. Space plays, ← → step a frame (shift: ten). mean / max collapse the recording, AVG averages that many frames around the slider, ↕ MESc shows it upside down as MESc does (the picture only), PNG saves what is on screen.
Pick spot, rect or polygon and click the image (polygon: click the corners, double-click to close); r / w × h set the size of new ones. Drag an ROI to move it, ↑ ↓ select, Delete removes the selected one, double-click a name in the list to rename it. ruler measures in µm (Esc clears); the hand icon drags a zoomed view. copy from… takes the ROIs of another unit; ROIs ▾: clear all, the set as an ImageJ zip or as json. ROIs are kept beside the viewer, never in the raw file.
fixed: one window in the recording's units for every frame (starts at the file's own LUT, file brings it back); auto %: stretched on percentiles of the whole recording. brightness / contrast / gamma (slider or a typed number) change the picture on screen only — gamma below 1 lifts dim boutons.
They compute themselves half a second after the ROIs change (Enter recomputes). raw | dF/F — dF/F by the pipeline's method; baseline ▾ sets its rolling quantile and window. filter ▾: Gaussian or Savitzky–Golay smoothing of what is drawn — the csv and the exports stay unfiltered. stack | overlay; grid ▾: the grid on or off, minor ticks, fixed steps, the zero line; y limits typed, or auto. Drag a box zooms time (in overlay the values too), double-click shows the whole recording, ← → slide a zoomed window; a click puts the movie on that frame. export ▾: csv, or the traces as drawn as a PNG.
3D: drag turns, wheel comes closer; window ▾, view ▾, box ▾, measure ▾ hold the rest. XYZ: three cuts through one crosshair. mosaic: every stack with a stage position in one field — edit, auto-stitch, crop, TIFF.
Space play · ← → frame / slide (shift: ×10) · ↑ ↓ select ROI · Delete remove ROI · Enter recompute traces · Esc close a menu, else cancel the draft / ruler / last 3D point.
Menus (name ▾) open one at a time and close on Esc or a click outside; a menu with something switched on inside it is marked like a button that is on. The view and the ROIs save themselves.
Colours after NERV Theme by Jane Manchun Wong (MIT).