Metadata-Version: 2.1
Name: sgRNAble
Version: 1.0.12
Summary: CRISPR-Cas9 single guide RNA generation tool
Home-page: https://github.com/hallamlab/sgRNAble
Author: Avery Noonan, Siddarth Raghuvanshi, Ahmed Abdelmoneim
Author-email: sidr97@gmail.com
License: UNKNOWN
Description: # sgRNAble
        
        __sgRNAble__ is a tool for high-throughput design of sgRNA libraries targeting selected genes or whole genomes, while considering both on-target binding potential and off-target effects of a given sgRNA in a user-defined genome.
        
        ## Basic Usage
        
        ```
         sgrnable -t TARGET_SEQUENCE.fasta -g GENOME_SEQUENCE.fasta
                  [GENOME_SEQUENCE.fasta ...] [-a AZIMUTH_CUTOFF]
                  [-c COPY_NUMBER [COPY_NUMBER ...]]
                  [-o OUTPUT_DIR] [-p PURPOSE]
                  [-th NUM_THREADS] [-m MAX_MEMORY] [-v]
        ```
        
        ## Documentation
        
        All documentation for the current release is accessible through the [sgRNAble wiki](https://github.com/hallamlab/sgRNAble/wiki).
        
        ## Authors
        * [Siddarth Raghuvanshi](https://github.com/Siddarth-Raghuvanshi)
        * [Ahmed Abdelmoneim](https://github.com/AhmedAbdelmoneim)
        * [Avery Noonan](https://github.com/Noonanav)
        
        ## Contact
        
        Need something? Please contact Professor Steven Hallam and Avery Noonan at: shallam@mail.ubc.ca and avery.noonan@ubc.ca
        
        ## References
        
        Farasat, I., & Salis, H. M. (2016). A Biophysical Model of CRISPR/Cas9 Activity for Rational Design of Genome Editing and Gene Regulation. _PLOS Computational Biology_, __12__(1), e1004724. doi:10.1371/journal.pcbi.1004724
        
        Doench, J. G., Fusi, N., Sullender, M., Hegde, M., Vaimberg, E. W., Donovan, K. F., . . . Root, D. E. (2016). Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. _Nature biotechnology_, __34__(184). doi:10.1038/nbt.3437
        
Platform: UNKNOWN
Classifier: Programming Language :: Python :: 3
Classifier: Operating System :: OS Independent
Requires-Python: ==3.7.*
Description-Content-Type: text/markdown
